Transplant immuno-diagnostics: crossmatch and antigen detection

Human leukocyte antigen typing and crossmatch: A comprehensive review

Renal transplantation remains the best option for patients suffering from end stage renal disease (ESRD). Given the worldwide shortage of organs and growing population of patients with ESRD, those waitlisted for a transplant is ever expanding. Contemporary crossmatch methods and human leukocyte antigen (HLA) typing play a pivotal role in improving organ allocation and afford better matches to r...

Evaluation of an Immuno-Chromatographic Detection System for Shiga Toxins and the E. coli O157 Antigen

Living-donor kidney transplant in T-cell and B-cell flow cytometry crossmatch-positive patients.

OBJECTIVES Complement-dependent cytotoxic crossmatch is an important indicator for kidney transplant. However, there is controversy about treatment for flow cytometry crossmatch-positive cases. MATERIALS AND METHODS This was a retrospective study of 127 living-donor kidney transplant recipients from May 2007 to July 2011. We divided patients into 115 flow cytometry crossmatch T-cell and B-cel...

Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.

An antigen detection system, termed immuno-polymerase chain reaction (immuno-PCR), was developed in which a specific DNA molecule is used as the marker. A streptavidin-protein A chimera that possesses tight and specific binding affinity both for biotin and immunoglobulin G was used to attach a biotinylated DNA specifically to antigen-monoclonal antibody complexes that had been immobilized on mi...

Immuno-PCR for detection of antigen to Angiostrongylus cantonensis circulating fifth-stage worms.

BACKGROUND Definitive diagnosis of infestation with Angiostrongylus cantonensis is difficult because the parasitic nematode is undetectable in the cerebrospinal fluid (CSF) of one-half of afflicted patients and the diagnostic sensitivity of ELISA for circulating worm antigens in patient sera is low. We studied immuno-PCR as a diagnostic tool. METHODS We studied 30 controls and 60 afflicted pa...